- Obstetricians & Gynecologists, Gainesville - Ob-Gyn Doctors, Physicians & Surgeons (Obstetrics & Gynecology)
- Occupational Health Care Physicians & Surgeons, Gainesville - Occupational Medicine
- Occupational Health Care Professionals, Gainesville
- Off Road Vehicles, Gainesville
- Off Road Vehicles Parts & Accessories, Gainesville
- Office Supplies, Equipment & Office Furniture Stores, Gainesville
- Oil & Gas Exploration & Development, Gainesville - Oil Field Exploration, Monitoring & Remediation
- Oil & Gas Producers, Gainesville - Companies
- Oil & Gas Well Drilling Services, Gainesville
- Oil & Petroleum Products, Gainesville - Merchant, Wholesalers, etc
- Oil Change & Lubrication, Gainesville - Auto Oil & Quick Lube
- Oil Field Contractors, Gainesville
- Oil Industry & Oil Field Equipment & Services, Gainesville - Oil Companies
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- Oilfield Trucking, Gainesville
- Ophthalmologists, Gainesville - Eye Doctors, Physicians & Surgeons (Ophthalmology)
- Optical Stores - Eyeglasses, Lenses & Sunglasses, Gainesville
- Opticians, Gainesville
- Optometrists, Gainesville - Eye Doctors, Physicians & Surgeons (Optometry)
- Ornamental Nursery, Gainesville
- Ornamental Nursery Services, Gainesville
- Orthodontist, Gainesville - Teeth Straightening, Invisalign & Dental Braces
- Other Building Materials & Supplies, Gainesville
- Other Lodging, Gainesville
- Outdoor Equipment & Accessories, Gainesville
- Overhead Door, Gainesville
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